Hospital Pharmaceutics

Simultaneous quantification of approximately 20 acylcarnitines (ACs) containing their isomers has been being developed by usage of HPLC-MS/MS (Figure 1). This method can be applied to the newborn screening for organic acidemias etc. AC profiles have been examined in biopsied and autopsied tissues of the patients

Medium- and short-chain acyl-CoAs in organic samples have been quantified for the elucidation of correlations among diseases, drugs and fatty acid metabolism.

Toxic metabolites of 6-mercaptopurine in human erythrocyte saline solution have been investigated.

Figure 1. LC-MS/MS spectra of ACs obtained by MRM mode: 1, carnitine (C); 2, malonyl-C; 3, acetyl-C; 4, succinyl-C; 5, glutaryl-C; 6, methylmalonyl-C; 7, propionyl-C; 8, isobutyryl-C; 9, butyryl-C; 10, pivaloyl-C; 11, 2-methylbutyryl-C; 12, isovaleryl-C; 13, valeryl-C; 14, hexanoyl-C; 15, heptanoyl-C; 16, valproyl-C; 17, octanoyl-C.

Recent Publications

Simultaneous Quantification of Acylcarnitine Isomers Containing Dicarboxylic Acylcarnitines in Human Serum and Urine by High Performance Liquid Chromatog- raphy-Electrospray Ionization Tandem Mass Spectrometry, Rapid Commun. Mass Spectrometry, 21, 799-806 (2007).

Novel Mutations in the Cytochrome P450 2C19 Gene: A Pitfall of the PCR-RFLP Method for Identifying a Common Mutation, J. Hum. Genet., 51, 118-123 (2006).

Genetic Basis of Inosine Triphosphate Pyrophosphohydrolase Deficiency in the Japanese Population, Mol. Genet. Metab., 85, 271-279 (2005).

Esterase-Like Activity of Serum Albumin: Characterization of Its Structural Chemistry Using p-Nitrophenyl Esters as Substrates, Pharm. Res., 21, 285-292 (2004).