学生の募集
学生の募集
研究内容
研究内容
研究内容
研究内容





2022年

Teramoto H., Hirashima N., Tanaka M.
A simple method for purified primary culture of enteric glial cells from mouse small intestine.
Biol. Pharm. Bull. 42 , 547-551 (2022)


2021年

Horie Y., Arame T., Hirashima N., Tanaka M.
Promotion of dendritic differentiation of cerebellar Purkinje cells by Ca2+/calmodulin-dependent protein kinase IIα, IIβ and IV and possible involvement of CREB phosphorylation.
Neuroscience 458, 87-98 (2021)

Suzuki R., Inoh Y., Yokawa S., Furuno T., Hirashima N.
Receptor dynamics regulates actin polymerization state through phosphorylation of cofilin in mast cells.
Biochem. Biophys. Res. Commun. 534, 714-719 (2021)


2019年

Suzuki R., Inoh Y., Yokawa S., Suzuki R., Furuno T., Hirashima N.
Monomer hapten and hapten-specific IgG inhibit mast cell activation evoked by multivalent hapten with different mechanisms.
Eur. J. Immunol. 49, 2172-2183 (2019)

Okura U., Hirashima N., Tanaka M.
Calcineurin B1 deficiency in glial cells reduces gastrointestinal motility and results in maldigestion and/or malabsorption in mice.
Biol. Pharm. Bull. 42, 1230-1235 (2019)


2018年

Inoue Y., Hasegawa S., Miyachi K., Yamada T., Nakata S., Ipponjima S., Hibi T., Nemoto T., Tanaka M., Suzuki R., Hirashima N.
Development of 3D imaging technique of reconstructed human epidermis with immortalized human epidermal cell line.
Exp. Dermatol. 27, 563-570 (2018)

Fujita M., Yagi T., Okura U., Tanaka J., Hirashima N., Tanaka M.
Calcineurin B1 deficiency in glial cells induces mucosal degeneration and inflammation in mouse small intestine.
Biol. Pharm. Bull. 41, 786-796 (2018)


2017年

Tanaka M., Senda T., Hirashima N.
Expression of the GluA2 subunit of glutamate receptors is required for the normal dendritic differentiation of cerebellar Purkinje cells.
Neurosci. Lett. 657, 22-26 (2017)

Yokawa S., Suzuki T., Inoue S., Inoh Y., Suzuki R., Kanamori T., Furuno T., Hirashima N.
Visualization of glucagon secretion from pancreatic α cells by bioluminescence video microscopy: identification of secretion sites in the intercellular contact regions.
Biochem. Biophys. Res. Commun. 485, 725-730 (2017)


2016年

Yokawa S., Furuno T., Suzuki T., Inoh Y., Suzuki R., Hirashima N.
Effect of cell adhesion molecule 1 expression on intracellular granule movement in pancreatic α cells.
Cell Biochem. Biophys. 74, 391-398 (2016)

Tadokoro S., Shibata T., Inoh Y., Amano T., Nakanishi M., Hirashima N, Utsunomiya-Tate N.
Phosphorylation of syntaxin-3 at Thr 14 negatively regulates exocytosis in RBL-2H3 mast cells.
Cell Biol. Int. 40, 589-596 (2016)

Tadokoro S., Hirashima N., Utsunomiya-Tate N.
Effect of complexin II on membrane fusion between liposomes containing mast cell SNARE proteins.
Biol. Pharm. Bull. 39, 446-449. (2016)

鈴木 亮
IgE受容体による多彩な抗原認識とアレルギー応答制御機構
アレルギー・免疫 277, 32-39 (2016)



2015年

Suzuki R., Scheffel J., Rivera J.
New insights on the signaling and function of the high affinity receptor for IgE.
Curr. Top. Microbiol. Immunol. 388, 63-90 (2015)

Tadokoro S., Inoh Y., Nakanishi M., Hirashima N.
Effects of PIP2 on membrane fusion between mast cell SNARE liposomes mediated by synaptotagmin 2.
Biochem. Biophys. Acta (Biomembrane) 1848, 2290-2294 (2015)


Tanaka M.
The dendritic differentiation of Purkinje neurons: Unsolved mystery in formation of unique dendrites.
Cerebellum 14, 227-230 (2015)

鈴木 亮
アレルゲン親和性によるマスト細胞活性化機構
臨床免疫・アレルギー科 64, 423-428 (2015)

鈴木 亮
IgEの高親和性受容体による分子レベルでの細胞応答制御
Science Booklet, p27 (2015)


2014年

Suzuki R., Leach S., Liu W., Ralston E., Scheffel J., Zhang W., Lowell CA., Rivera J.
Molecular editing of cellular responses by the high-affinity receptor for IgE.
Science 343, 1021-1025 (2014)

Rivera J., Dema B., Suzuki R.
Rethinking the role of immunoglobulin E and its high affinity receptor: New insights in allergy and beyond.
Int. Arch. of Allergy Immunol. 164, 271-279 (2014)

Ikeya M., Yamanoue K., Mochizuki Y., Konishi H., Tadokoro S., Tanaka M., Suzuki R., Hirashima N.
Orai-2 is localized on secretory granules and regulates antigen-evoked Ca2+ mobilization and exocytosis in mast cells.
Biochem. Biophys. Res. Commun. 451, 62-7 (2014)


Inoue Y., Hasegawa S., Ban S., Yamada T., Date Y., Mizutani H., Nakata S., Tanaka M., Hirashima N.
ZIP2, a zinc transporter, is associated with keratinocyte differentiation.
J. Biol. Chem. 289, 21451-62 (2014)


Nishikawa S., Hirashima N., Tanaka M.
Optimization of single-cell electroporation protocol for forced gene expression in primary neuronal cultures.
Mol. Biotechnol. 56, 824-832 (2014)

Ohashi R., Sakata S., Naito A., Hirashima N., Tanaka M.
Dendritic differentiation of cerebellar Purkinje cells is promoted by ryanodine receptors expressed by Purkinje and granule cells.
Dev. Neurobiol. 74, 467-480 (2014)


2013年

Inoh Y., Tadokoro S., Tanabe H., Inoue M., Hirashima N., Nakanishi M., Furuno T.
Inhibitory effects of a cationic liposome on allergic reaction mediated by mast cells activation.
Biochem. Pharmacol. 86, 1731-8 (2013)

Inoh Y., Furuno T., Hirashima N., Kitamoto D., Nakanishi M.
Synergistic effect of a biosurfactant and protamine on gene transfesciton efficiency.
Eur. J. Pharm. Sci. 49, 1-9 (2013)

平嶋尚英、田所 哲
巨大リポソームによるエンド/エクソサイトーシス系のモデル開発
生物物理 53, 98-100 (2013)



2012年

Furuno T., Hagiyama M., Sekimura M., Okamoto K., Suzuki R., Ito A., Hirashima N., Nakanishi M.
Cell adhesion molecule 1 (CADM1) on mast cells promotes interaction with dorsal root ganglion neurites by heterophilic binding to nectin-3.
J. Neuroimmunol. 250, 50-58 (2012)

Takekawa M., Furuno T., Hirashima N., Nakanishi M.
Mitochondria take up Ca2+ in two steps dependently on store-operated Ca2+ entry in mast cells.
Biol. Pharm. Bull. 35, 1354-1360 (2012)

Tahara K., Tadokoro S., Kawashima Y., Hirashima N.
Endocytosis-like uptake of surface-modified drug nano-carriers into giant unilamellar vesicles.
Langmuir 28, 7114-7118 (2012)

Tahara K., Tadokoro S., Yamamoto H., Kawashima Y., Hirashima N.
The suppression of IgE-mediated histamine release from mast cells following exocytic exclusion of biodegradable polymeric nanoparticles.
Biomaterials 33, 343-351 (2012)

Nishimura Y., Tadokoro, S., Tanaka M., Hirashima, N.
Detection of asymmetric distribution of phospholipids by fluorescence resonance energy transfer.
Biochem. Biophys. Res. Commun. 420, 926-930 (2012)

Tanaka M.
Single-cell electroporation of siRNA in primary neuronal cultures.
In: Controlled Genetic Manipulations, Neuromethods 65, 129-139, Springer, New York (2012)


2011年


Inoh Y., Furuno T., Hirashima N., Nakanishi M.
Rapid delivery of small interfering RNA by biosurfactant MEL-A-containing liposomes.
Biochem. Biophys. Res. Commun. 414, 635-640 (2011)

Morita Y., Tadokoro S., Sasai M., Kitamoto D., Hirashima N.
Biosurfactant mannosyl-erythritol lipid inhibits secretion of inflammatory mediators from RBL-2H3 cells.
Biochim. Biophys. Acta 1810, 1302-1308 (2011)

Nagai Y., Tadokoro S., Sakiyama H., Hirashima N.
Effects of synaptotagmin 2 on membrane fusion between liposomes that contain SNAREs involved in exocytosis in mast cells.
Biochim. Biophys. Acta 1808, 2435-2439 (2011)

Nomura H., Tadokoro S., Hirashima N.
Liprin-alpha is involved in exocytosis and cell spreading in mast cells.
Immunol. Lett. 139, 110-116 (2011)

Tanaka M., Asaoka M., Yanagawa Y., Hirashima N.
Long-term gene-silencing effects of siRNA introduced by single-cell electroporation into postmitotic CNS neurons.
Neurochem. Res. 36, 1482-1489 (2011)

Tahara K., Sakai T., Yamamoto H., Takeuchi H., Hirashima N., Kawashima Y.
Improvements in transfection efficiency with chitosan modified poly(DL-lactide-co-glycolide) nanospheres prepared by the emulsion solvent diffusion method for gene delivery.
Chem. Pharm. Bull. 59, 298-301 (2011)

Miyata M., Kishimoto Y., Tanaka M., Hashimoto K., Hirashima N., Murata Y., Kano M., Takagishi Y.
A role for myosin Va in cerebellar plasticity and motor learning: a possible mechanism underlying neurological disorder in myosin Va disease.
J. Neurosci. 31, 6067-6078 (2011)

平嶋尚英、田所 哲
マスト細胞の脱顆粒とSNARE関連分子
臨床免疫・アレルギー科 51, 617-622 (2011)



2010年

Sasai M., Tadokoro S., Hirashima N.
An artificial exocytotic system that secretes intravesicular contents upon Ca2+ influx.
Langmuir 26, 14788-14792 (2010)

Hiramatsu H., Tadokoro S., Hirashima N.
Latrotoxin induces degranulation in RBL-2H3 cells transfected with latrophilin.

Toxicon 56, 1372-1380 (2010)

Inoh Y., Furuno T., Hirashima N., Kitamoto D., Nakanishi M.
The ratio of unsaturated fatty acids in biosurfactats affects the efficiency of gene transfection.
Int. J. Pharm. 398, 225-230 (2010)


Hoshino Y., Hirashima N., Nakanishi M., Furuno T.
Inhibition of degranulation and cytokine production in bone marrow-derived mast cells by hydrolyzed rice bran.
Inflamm. Res. 59, 615-625 (2010)


Kawamura F., Nakanishi M., Hirashima N.
Effects of menadione, a reactive oxygen generator, on leukotriene secretion from RBL-2H3 cells.
Biol. Pharm. Bull. 33, 881-885 (2010)

Tahara K., Sakai T., Yamamoto H., Takeuchi H., Hirashima N., Kawashima Y.
Chitosan modified poly (D,L-lactide-co-glycolide) nanospheres for improving siRNA delivery and gene silencing effects.
Eur. J. Pharmaceut. Biopharmaceut. 74, 421-426 (2010)


Tadokoro S., Nakanishi M., Hirashima N.
Complexin II regulates degranulation in RBL-2H3 cells by interacting with SNARE complex containing syntaxin-3.
Cell. Immunol. 261, 51-56 (2010).

田中正彦
培養神経細胞におけるGFPイメージングを用いた単一細胞レベルでのRNA干渉効果の解析.
バイオイメージング
19, 9-13 (2010)


平嶋尚英
アレルギー治療薬.
看護技術 56, 75-80 (2010)

平嶋尚英、田所 哲
リポソームを用いたSNARE複合体介在膜融合のモデル.
生体の科学 61, 204-208 (2010)



2009年

Tahara K., Sakai T., Yamamoto H., Takeuchi H., Hirashima N., Kawashima Y.
Improved cellular uptake of chitosan-modified PLGA nanospheres by A549 cells.
Int. J. Pharm. 382, 198-204 (2009)


Tanaka M., Sakata S., Hirashima, N.
Effects of 1-naphthyl acetyl spermine on dendrite formation by cultured cerebellar Purkinje cells.
Neurosci. Lett. 462, 30-32 (2009)

Nomura H.,Ohtsuka T., Tadokoro S., Tanaka M., Hirashima N.
Involvement of ELKS, an active zone protein, in exocytotic release from RBL-2H3 cells.
Cell. Immunol. 258, 204-211 (2009)


Tanaka M.
Dendrite formation of cerebellar Purkinje cells.
Neurochem. Res. 34, 2078-2088 (2009)

Tanaka M., Yanagawa Y., Hirashima N.
Transfer of small interfering RNA by single-cell electroporation in cerebellar cell cultures.
J. Neurosci. Meth. 178, 80-86 (2009)


Inoh Y., Furuno T., Nakanishi M., Hirashima N.
Non-viral vectors with a biosurfactant MEL-A promote gene transfection into solid tumors in mouse abdominal cavity.
Biol. Pharm. Bull. 32, 126-128 (2009)

Sakiyama H., Tadokoro S., Nakanishi M., Hirashima N.
Membrane fusion between liposomes containing SNARE proteins involved in mast cell exocytosis.
Inflamm. Res. 58, 139-142 (2009)


2008年

Miura K., Nomura H., Nakanishi M., Hirashima N.

Munc13-1, an active zone protein, regulates exocytosis in mast cell.
Bioimages 16, 11-18 (2008)


2007年

Chikamatsu S., Furuno T., Kinoshita Y., Hirashima N.,
Teshima R., Nakanishi M.
Effects of Cot expression on the nuclear translocation of NF-kB in RBL-2H3 cells.
Mol. Immunol. 44, 1490-1497 (2007)


Ueno Y., Hirashima N., Inoh Y., Furuno T., Nakanishi M.
Characterization of biosurfactant-containing liposomes and their transfection efficiency for gene transfection.
Biol. Pharm. Bull. 30, 169-172 (2007)

Hirashima N., Minatani K., Hattori Y., Ohwada T., Nakanishi M.
Anchoring cationic amphiphiles for nucleotide delivery significance of DNA release from cationic liposomes for transfection.
Biol. Pharm. Bull. 30, 1117-1122 (2007)

Tadokoro S., Kurimoto T., Nakanishi M., Hirashima N.
Munc18-2 regulates exocytotic membrane fusion positively interacting with syntaxin 3 in RBL-2H3 cells.
Mol. Immunol. 44, 3427-3433 (2007)


Tanaka M.
Role of a chondroitin sulfate proteoglycan PTPζ in dedrite development of
cerebellar Purkinje cells.
In: Neural Proteoglycan, pp. 153-166, Research Signpost, Kerala, India (2007)


Tanaka M., Kokubo M., Marunouchi T.
Asymmetric localization of Notch2 on the microvillous surface in choroid plexus epithelial cells.
Histochem. Cell Biol. 127, 449-456 (2007)

Ueno Y., Inoh Y., Furuno T., Hirashima N., Kitamoto D., Nakanishi M.
NBD-conjugated biosurfactant (MEL-A) shows a new pathway for transfection.
J. Control. Release 123, 247-253 (2007)

平嶋尚英、加藤直人
マスト細胞におけるFceレセプターIシグナルによるLynの活性化機序.
臨床免疫アレルギー 47, 408-413 (2007)

田中正彦
小脳プルキンエ細胞樹状突起の形態形成のメカニズム:受容体型チロシンホスファターゼPTPζの役割を中心として.
日本神経精神薬理学雑誌
27, 135-140 (2007)



2006年

Kato N., Nakanishi M., Hirashima N.
Flotillin-1 regulates IgE receptor-mediated signaling in RBL-2H3 cells.
J. Immunol. 177, 147-154 (2006)


Tanaka M., Duncan R. S., McClung N., Yannazzo J. -A, S., Marunouchi T., Inokuchi K., Koulen P.
Homer proteins control neuronal differentiation through IP
3 receptor signaling.
FEBS Lett. 580, 6145-6150 (2006)

Tanaka M., Yanagawa Y., Obata K., Marunouchi T.
Dendritic morphogenesis of cerebellar Purkinje cells through extension and retraction revealed by long-term tracking of living cells in vitro.

Neuroscience 141, 663-674 (2006)


Kawamura F., Hirashima N., Furuno T., Nakanishi M.
The Effects of 2-methyl-1,4-naphtoquinone (Menadione) on Cellular Signaling in RBL-2H3 Cells.

Biol. Pharm. Bull. 29, 605-607 (2006).


2005年

Tadokoro S., Nakanishi M.,Hirashima N.
Complexin II facilitates exocytotic release positively by enhancing Ca2+ sensitivity of fusion process in mast cells.
J. Cell Sci. 118, 2239-2246 (2005).


Tanaka M., Marunouchi T.
Abnormality in the cerebellar folial pattern of C57BL/6J mice.
Neurosci. Lett. 390, 182-186 (2005)

Shimazaki Y., Nagata I., Tanaka M., Marunouchi T., Hata T., Maeda N.
Developmental change and function of chondroitin sulfate deposited around cerebellar Purkinje cells.
J. Neurosci. Res. 82, 172-183 (2005)

田中正彦
神経樹状突起の形成メカニズム.
Priming BioMedicine 2, 23-35 (2005)


2004年

Inoh Y., Kitamoto D., Hirashima N., Nakanishi M.
Biosurfactant MEL-A dramatically increases gene transfection via membrane fusion.

J. Control. Release 94, 423-431 (2004)



2003年

Noguchi S., Hirashima N., Furnuno T., Nakanishi M.
Remarkable induction of apoptosis in human cancer cells by a new cationic liposome complexed with a bcl-2 antisense oligonucleotide.
J. Control. Release 88, 313-320 (2003)


Noguchi S., Hirashima N., Nakanishi M.
Asialoganglioside Enhances the Efficiency of Gene Transfection Mediated by Cationic Liposomes with a Cationic Cholesterol Derivative
Biol. Pharm. Bull. 26, 1306-1310 (2003)

Kato N., Nakanishi M., Hirashima N.
Cholesterol depletion inhibits store-operated calcium currents and exocytotic membrane fusion in RBL-2H3 cells
Biochemistry 42, 11808-11814 (2003)

Ohyama N., Furuno T., Hirashima N., Nakanishi M.
The effects of ITIM-bearing FcRIIB on the nuclear shuttling of MAP kinase in RBL-2H3 cells
Immunol. Lett. 90, 173-176 (2003)

Tanaka M., Marunouchi T.
Immunohistochemical localization of Notch receptors and their ligands in the postnatally developing rat cerebellum.
Neurosci. Lett. 353, 87-90 (2003)


Tanaka M., Maeda N., Noda M., Marunouchi T.
A chondroitin sulfate proteoglycan PTPζ/RPTPβ regulates the morphogenesis of Purkinje cell dendrites in the developing cerebellum.
J. Neurosci. 23, 2804-2814 (2003)



2002年

Noguchi A., Hirashima N., Nakanishi M.
Cationic cholesterol promotes gene transfection using the nuclear localization signal in protamine.
Pharmaceut. Res. 19, 933-938 (2002)


Noguchi A., Hirashima N., Furuno T., Nakanishi M.
Activation of receptor tyrosine kinases promotes gene transfection in rat neuronal PC12 cells by cationic liposomes.
.
Neurosci. Lett. 325, 29-32 (2002)

Kato N., Nakanishi M., Hirashima N.
Transbilayer asymmetry of phospholipids in the plasma membrane regulates exocytotic release in mast cells.
Biochemistry 41, 8068-8074 (2002)


Hasegawa S., Hirashima, N., Nakanishi, M.
Comparative Study of Transfection Efficiency of Cationic Cholesterols Mediated by Liposomes-based Gene Delivery.
Bioorg. Med. Chem. Lett. 12, 1299-1302 (2002)



2001年

Fujiwara T., Hirashima N., Hasegawa S., Nakanishi M., Ohwada T.
Space-filling Effects in Membrane Disruption by Cationic Amphiphiles.
Bioorg. Med. Chem. 9, 1013-1024 (2001)


Furuno T., Hirashima N., Sagiya N., Nakanishi M.
Nuclear shuttling of MAP kinase (ERK2) was dynamically controlled by MEK after antigen stimulation in RBL-2H3 cells.
J. Immunol. 166, 4416-4421 (2001)

Satoh Y., Hirakura Y., Shibayama S., Hirashima N., Kirino Y.
β-Amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from the electric ray.
Neurosci. Lett. 302, 97-100 (2001)

Amano T., Furuno T., Hirashima N., Ohyama N., Nakanishi M.
Dynamics of intracellular granules with CD63-GFP in rat basophilic leukemia cells.
J. Biochem. 129, 739-744 (2001)

Hasegawa S., Hirashima N., Nakanishi M.
Microtubule involvements in the intracellular dynamics for gene transfection mediated by cationic liposomes.
Gene Therapy 8, 1669-1673 (2001)

Inoh Y., Kitamoto D., Hirashima N., Nakanishi M.
Biosurfactants of MEL-A increase gene transfection mediated by cationic liposomes.
Biochem. Biophys. Res. Commun. 289, 57-61 (2001)

Yoshimura T., Hasegawa S., Hirashima N., Nakanishi M., Ohwada T.
Anchoring and bola cationic amphiphiles for nucleotide delivery.  Effects of orientation and expression of hydrophobic regions.
Bioorg. Med. Chem. Lett. 11, 2897-2901 (2001)

中西 守, 平嶋尚英
SNAREモチーフ.
生体の科学 52, 418-419 (2001)


田中正彦
小脳切片培養系を用いた神経系細胞の増殖・移動・分化・細胞死の機構解析.
神経化学
40, 566-582 (2001)



2000年

Hibi T., Hirashima N., Nakanishi M.
Rat basophilic leukemia cells express syntaxin-3 and VAMP-7 in granule membranes.
Biochem. Biophys. Res. Commun. 271, 36-41 (2000).


Hirashima N., Nishio M.,Nakanishi M.
Intracelluar dynamics of a high affinity NGF receptor TrkA in PC12 cell.
Biol. Pharm. Bull. 23, 1097-1099 (2000)

Fujiwara T., Hasegawa S., Hirashima N., Nakanishi M., Ohwada T.
Gene transfection activities of novel amphiphilic cholestane-polyamine conjugates.
Biochim. Biophys. Acta 1468, 396-402 (2000)

Lee D., Hirashima N., Kirino Y.
Rapid transbilayer phospholipid redistribution associated with exocytotic release of neurotransmitters from cholinergic nerve terminals isolated from electric ray Narke japonica
Neurosci. Lett. 291, 21-24 (2000)

Tanaka M., Momoi T., Marunouchi T.
In situ detection of activated caspase-3 in apoptotic granule neurons in the developing cerebellum in slice cultures and in vivo.

Dev. Brain Res. 121, 223-228 (2000)

平嶋尚英
神経・免疫系におけるエクソサイトーシスの分子機構.
薬学雑誌 120, 1419-1427 (2000)